Elster Online 2013 Download: A Step-by-Step Guide

Introduction

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In the field of biotechnology, micro-algae have gained significant attention due to their ability to produce a wide range of valuable compounds. Among these, Chlorella mirabilis is an Antarctic soil green alga that has been found to possess unique characteristics that make it an attractive candidate for biotechnological applications. In this article, we will explore the process of micro-scale mass cultivation of Chlorella mirabilis and discuss the importance of nitrogen and carbon sources in promoting its growth.

Micro-Scale Mass Cultivation

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The micro-scale mass cultivation of Chlorella mirabilis was performed using a stable (in-door) and variable (out-door) environment during winter and early spring in the Czech Republic. The treatments used to determine the effects of different nitrogen and carbon sources on the alga's growth included:

* Pure Z medium (control, Z)

* Z medium + 5% glycerol (ZG)

* Z medium + 5% glycerol + 50 μM KNO3 (ZGN)

* Z medium + 5% glycerol + 200 μM NH4Cl (ZGA)

* Z medium + 5% glycerol + 1 mM Na2CO3 (ZNC)

* Z medium + 5% glycerol + 1 mM Na2CO3 + 200 μM NH4Cl (ZGCA)

* Z medium + 5% glycerol + 1 mM Na2CO3 + 50 μM KNO3 (ZGCN)

These treatments were performed at a temperature of 15°C with an irradiance of 75 μmol m−2 s−1. The results showed that the alga was able to adapt and grow well in all the treatment conditions, indicating its potential for mass cultivation.

Nitrogen and Carbon Sources

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The availability of nitrogen and carbon sources is crucial for the growth of micro-algae like Chlorella mirabilis. In this study, we evaluated the effects of different nitrogen and carbon sources on the alga's growth characteristics. The results showed that the addition of glycerol to the medium improved the growth rate of the alga, while the addition of Na2CO3 had a negative effect on its growth.

Fatty Acid Contents

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In addition to evaluating the effects of different nitrogen and carbon sources on the alga's growth characteristics, we also analyzed the fatty acid contents of Chlorella mirabilis. The results showed that the alga contained a high amount of unsaturated fatty acids, which are valuable compounds with potential applications in biotechnology.

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In conclusion, micro-scale mass cultivation of Chlorella mirabilis is a promising approach for producing valuable compounds like fatty acids. The availability of nitrogen and carbon sources plays a crucial role in promoting the growth of this alga. Further studies are needed to optimize the conditions for its mass cultivation and to evaluate its potential applications in biotechnology.

References

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* 4Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Centre AS CR, České Budějovice, Czech Republic

* 3Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic

* 2Centre for Phycology, Institute of Botany AS CR, Třeboň, Czech Republic

* 1Aquatic Environment Management Division, Central Institute of Fisheries Education, Mumbai, India

Elster online 2013 download